Journal
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
Volume 372, Issue 1726, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rstb.2016.0214
Keywords
computational design; membrane protein folding; metallotransporter
Categories
Funding
- NIH [F32 GM096727, R01 GM054616]
- NSF [CHE-141329, DMR1120901]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1413295] Funding Source: National Science Foundation
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Ion transporters and channels are able to identify and act on specific substrates among myriads of ions and molecules critical to cellular processes, such as homeostasis, cell signalling, nutrient influx and drug efflux. Recently, we designed Rocker, a minimalist model for Zn2+/H+ co-transport. The success of this effort suggests that de novo membrane protein design has now come of age so as to serve a key approach towards probing the determinants of membrane protein folding, assembly and function. Here, we review general principles that can be used to design membrane proteins, with particular reference to helical assemblies with transport function. We also provide new functional and NMR data that probe the dynamic mechanism of conduction through Rocker.
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