Journal
PHARMACEUTICAL BIOLOGY
Volume 55, Issue 1, Pages 1521-1527Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2017.1304427
Keywords
Antifungal agents; natural products; Caatinga biome
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [10.13039/501100003593]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [10.13039/501100002322]
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco [10.13039/501100006162]
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Context: Buchenavia tetraphylla (Aubl.) RA Howard (Combretaceae: Combretoideae) is an ethnomedicinal plant with reported antifungal action. Objective: This study evaluates the antimicrobial activity of B. tetraphylla leaf extracts against clinical isolates of Candida albicans. The morphological alterations, combinatory effects with fluconazole and the cytotoxicity of the active extract were analyzed. Materials and methods: Extracts were obtained using different solvents (hexane: BTHE; chloroform: BTCE; ethyl acetate: BTEE; and methanol: BTME). Antimicrobial activity was determined by the broth microdilution method using nine strains of C. albicans isolated from vaginal secretions and one standard strain (UFPEDA 1007). Results: All extracts showed anti-C. albicans activity, including against the azole-resistant strains. The MIC values ranged from 156 to 2500 mu g/mL for the BTHE; 156 to 1250 lg/mL for the BTCE; 625 to 1250 mu g/mL for the BTME and 625 mu g/mL to 2500 mu g/mL for the BTEE. BTME showed the best anti-C. albicans activity. This extract demonstrated additive/synergistic interactions with fluconazole. Scanning electron microscopy analysis suggested that the BTME interferes with the cell division and development of C. albicans. BTME showed IC50 values of 981 and 3935 mu g/mL, against J774 macrophages and human erythrocytes, respectively. This extract also enhanced the production of nitric oxide by J774 macrophages. Discussion and conclusion: Buchenavia tetraphylla methanolic extract (BTME) is a great source of antimicrobial compounds that are able to enhance the action of fluconazole against different C. albicans strains; this action seems related to inhibition of cell division.
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