Journal
PHARMACEUTICAL BIOLOGY
Volume 55, Issue 1, Pages 1185-1194Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2017.1291690
Keywords
Total phenolic content; flavonoids content; condensed tannins content; DPPH assay; RPC assay; Pistacia atlantica leaves
Ask authors/readers for more resources
Context: The widespread use of Pistacia atlantica Desf. ssp. (Anacardiaceae) in traditional medicine can be partly attributed to the content of its secondary metabolites, in particular, the phenolic compounds. Objective: The effects of harvest period, growing region and gender on the phenolic compounds, flavonoids and condensed tannins contents were studied, as well as on the antioxidant activities of P. atlantica leaves in order to provide a scientific basis for optimal collection. Materials and methods: Leaves were collected monthly from April to October 2010 in two Algerian sites. The powdered leaves were used for preparing the ethyl acetate extract. Contents of total phenolics (TPC), flavonoids (FC) and condensed tannins (CTC) were determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity (RSA) of 2,2-diphenyl-1-picrylhydrazyl (250 mu M) and the reducing power capacity (RPC) determination by K3Fe(CN)(6) (1%). Results: The TPC was found to vary from 79 +/- 13 to 259 +/- 8 mg gallic acid equivalents/g of dry weight (DW) during the study period. The RSA and RPC varied between 262 +/- 18 and 675 +/- 21 mg Ascorbic Acid Equivalent (AAE)/g DW, and from 259 +/- 16 to 983 +/- 20 mg AAE/g DW, respectively. A seasonal pattern was observed consisting of a decrease in TPC content and RPC from spring to autumn. The FC, CTC and RSA did not show a seasonal pattern. Discussion and conclusion: Our findings showed that secondary metabolite content and antioxidant activities of P. atlantica leaves were more influenced by harvest time and growing region than by gender.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available