Journal
PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING
Volume 62, Issue 3, Pages 257-264Publisher
BUDAPEST UNIV TECHNOLOGY ECONOMICS
DOI: 10.3311/PPch.10841
Keywords
Daphnia magna; heartbeat rate; micropollutants; triclosan
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Funding
- National Innovation Office (CDFILTER project) [TECH_08-A4/2-2008-0161]
- National Innovation Office (SOILUTIL project) [TECH_09-A4-2009-0129]
- New Hungary Development Plan [TAMOP-4.2.1/B-09/1/KMR-2010-0002]
- NATIONAL EXCELLENCE PROGRAM OF THE MINISTRY OF HUMAN CAPACITIES
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Development of an unconventional test method involves usually the comparison of biological responses under a variety of test conditions. The quality of these biological methods relies on an appropriate experimental design. The Daphnia magna heartbeat rate as a physiological endpoint for assessing aquatic pollution has been of minor interest so far; nonetheless, this could be an early and sensitive indicator of the harmful effect of micropollutants. Our aim was to set up the optimal experimental design of the heartbeat rate test. The studied factors were the composition of the test medium, the age of the test organism, and the exposure time, at triclosan concentrations between 0.2-2000 mu g/L. According to the evaluation of test results the optimal test condition for the heartbeat rate test assumes tap water as test medium, 10-day-old test organisms and 48 h exposure time.
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