Journal
PEDIATRIC SURGERY INTERNATIONAL
Volume 33, Issue 10, Pages 1065-1071Publisher
SPRINGER
DOI: 10.1007/s00383-017-4138-8
Keywords
Tracheal reconstruction; Decellularized trachea; Porcine model; Tissue-engineering; Regeneration
Categories
Funding
- JSPS KAKENHI [JP16K20348, JP16K11359]
- National Center for Child Health and Development [27-8]
- Grants-in-Aid for Scientific Research [16K11359, 17H04355] Funding Source: KAKEN
Ask authors/readers for more resources
Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 x 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available