Journal
ACS APPLIED BIO MATERIALS
Volume 1, Issue 3, Pages 777-782Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.8b00235
Keywords
fluorescence; carbon dots; MnO2 nanosheets; apoferritin; trypsin; fluorescence resonance energy transfer
Funding
- Beijing National Science Foundation [2174085]
- National Natural Science Foundation of China [21775089, 21705151]
- Outstanding Youth Foundation of Shandong Province [ZR2017JL010]
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A novel turn-on fluorescent trypsin detection platform dependant on carbon dot-MnO2 (CD-MnO2) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO2 nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.
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