Journal
BIOACTIVE MATERIALS
Volume 3, Issue 2, Pages 167-173Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2018.01.005
Keywords
Cell sheet engineering; Human mesenchymal stem cell; Polydimethylsiloxane; Layer-by-layer grafting; Cell adhesion
Funding
- National Institutes of Health [1R15CA202656]
- National Science Foundation [1703570]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1703570] Funding Source: National Science Foundation
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Human mesenchymal stem cell (hMSC) sheets hold great potential in engineering three-dimensional (3D) completely biological tissues for diverse applications. Conventional cell sheet culturing methods employing thermoresponsive surfaces are cost ineffective, and rely heavily on available facilities. In this study, a cost-effective method of layer-by-layer grafting was utilized for covalently binding a homogenous collagen I layer on a commonly used polydimethylsiloxane (PDMS) substrate surface in order to improve its cell adhesion as well as the uniformity of the resulting hMSC cell sheet. Results showed that a homogenous collagen I layer was obtained via this grafting method, which improved hMSC adhesion and attachment through reliable collagen I binding sites. By utilizing this low-cost method, a uniform hMSC sheet was generated. This technology potentially allows for mass production of hMSC sheets to fulfill the demand of thick hMSC constructs for tissue engineering and biomanufacturing applications. (c) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
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