Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 29, Issue 1, Pages 498-510Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2017.1406461
Keywords
Immunochromatographic assay; monoclonal antibody; mycotoxin; strip test; zearalenone
Funding
- National Key R D Program [2016YFD0401101]
- Natural Science Foundation of Jiangsu Province
- MOF [BE2016307, BK20150145, BX20151038, BK20140003, BE2014672, CMB21S1614, 201513006]
- Taishan Industry Leading Talent Special Funds
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Zearalenone (ZEN) is a mycotoxin produced by fungal species which are widespread in nature and commonly contaminate many cereal grains, such as wheat, maize, corn, barley, and other cereal grains. An immunochromatographic (ICA) test strip was developed for the rapid simultaneous detection of ZEN and its metabolite in corn flour samples. For the ICA test, antigen (ZEN-BSA) and goat anti-mouse IgG were, respectively, drawn on nitrocellulose membrane as control line and test line. Under the optimized condition, the cut-off limits of test strips for ZEN, -ZAL, and -ZAL were found to be 0.5ng/mL in 0.01M PBS and 50ng/mL in corn flour samples, meanwhile -ZOL, -ZOL, and ZEA were found to be 1ng/mL in 0.01M PBS and 75ng/mL in corn flour samples. Our data indicate that the ICA is sensitive, rapid, cost-effective, and specific on-site screening for ZEN and its metabolite.
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