Journal
PATHOBIOLOGY
Volume 85, Issue 3, Pages 192-200Publisher
KARGER
DOI: 10.1159/000481890
Keywords
IQGAP3; Mucin phenotype; Spheroid; Cancer stem cell; Gastric cancer
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Funding
- Japan Society for the Promotion of Science [B-15H04713, 26670175, 16K15247]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [16H06999] Funding Source: KAKEN
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Objective: Spheroid colony formation is a useful method of cancer stem cell (CSC) characterization. We previously showed that the IQ motif containing the GTPase-activating protein 3 gene (IQGAP3) is upregulated in spheroid body-forming gastric cancer (GC) cells compared with parental cells. We investigated IQGAP3 expression in GC. Methods: IQGAP3 protein expression was analyzed by immunohistochemistry in 165 GC cases. RNA interference was used to inhibit IQGAP3 expression in GC cell lines. Results: In nonneoplastic gastric mucosa, weak staining of IQGAP3 was observed in the foveolar epithelium, while GC tissue showed stronger, more extensive staining. Of the 165 GC cases, 34 (21%) were positive for IQGAP3 expression. GC cases positive for IQGAP3 were found more frequently in stage II/III/IV cases than in stage I cases. Univariate and multivariate analyses demonstrated that IQGAP3 expression is an independent prognostic classifier of GC patients. Both the number and size of the spheres formed by MKN-74 cells were significantly reduced by knockdown of IQGAP3. The phosphorylation of Akt and Erk1/2 was inhibited by knockdown of IQGAP3. Conclusion: These results suggest that IQGAP3 plays an important role in gastric CSCs. The location of IQGAP3 on the cell membrane makes it a potential therapeutic target for GC. (C) 2017 S. Karger AG, Basel
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