Phytochemical Study of the Ecuadorian Species Lepechinia mutica (Benth.) Epling and High Antifungal Activity of Carnosol against Pyricularia oryzae

The plant Lepechinia mutica (Benth.) Epling (family Lamiaceae) is endemic to Ecuador. In the present study, we report some major non-volatile secondary metabolites from the leaves and the chemistry of the essential oil distilled from the flowers. The main identified compounds were carnosol, viridiflorol, ursolic acid, oleanolic acid, chrysothol, and 5-hydroxy-4,7-dimethoxy flavone. Their structures were determined by X-ray diffraction and NMR and MS techniques. The essential oil showed a chemical composition similar to that distilled from the leaves, but with some qualitative and quantitative differences regarding several minor compounds. The main constituents (>4%) were: -3-carene (24.23%), eudesm-7(11)-en-4-ol (13.02%), thujopsan-2--ol (11.90%), -pinene (7.96%), valerianol (5.19%), and co-eluting limonene and -phellandrene (4.47%). The volatile fraction was also submitted to enantioselective analysis on a -cyclodextrin column, obtaining the separation and identification of the enantiomers for -thujene, -pinene, sabinene, -phellandrene, limonene and -phellandrene. Furthermore, the anti-fungal activity of non-volatile secondary metabolites was tested in vitro, with carnosol resulting in being very active against the blast disease caused by the fungus Pyricularia oryzae.