Journal
CONVERGENT SCIENCE PHYSICAL ONCOLOGY
Volume 4, Issue 1, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2057-1739/aaa013
Keywords
single cell analysis; imaging mass cytometry; CyTOF; protein biomarkers; HD-SCA; circulating tumor cells
Categories
Funding
- Breast Cancer Research Foundation [004698-00003]
- National Cancer Institute
- Leidos Biomedical Research, Inc [HHSN261200800001E]
- Prostate Cancer Foundation [16CHAL04]
- Vassiliadis Research Fellowship
- Polak Research Fellowship
- Vicky Joseph Research Fellowship
- Charles University Research Fund [Progres Q39]
Ask authors/readers for more resources
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established high definition single cell analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available