4.6 Article

Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

Journal

OPTICS LETTERS
Volume 42, Issue 2, Pages 227-230

Publisher

OPTICAL SOC AMER
DOI: 10.1364/OL.42.000227

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Funding

  1. National Natural Science Foundation of China (NSFC) [61405242]
  2. Cluster of Excellence of the Deutsche Forschungsgemeinschaft (DFG) [1003]
  3. Cluster of Excellence of the Deutsche Forschungsgemeinschaft (DFG) (DFG Cells in Motion) [EXC 1003]
  4. Visiting Scholarship for Young Scientists, Chinese Academy of Science

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We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method. (C) 2017 Optical Society of America

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