Journal
OPTICS LETTERS
Volume 42, Issue 4, Pages 659-662Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.42.000659
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Funding
- National Natural Science Foundation of China (NSFC) [81671725]
- Shanghai Rising Star Program [15QA1400500]
- Shanghai Action Plan for Scientific and Technological Innovation Program [16441909200]
- National Key Research and Development Program of China [2016YFA0301000, 2016YFC0102100]
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Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest. (C) 2017 Optical Society of America
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