Journal
OPTICS LETTERS
Volume 42, Issue 5, Pages 995-998Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.42.000995
Keywords
-
Categories
Funding
- National Science Foundation (NSF) [IIP-1068070]
- National Institutes of Health (NIH) [R01CA182939]
Ask authors/readers for more resources
We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOFmicroscopy are shown withGCaMPlabeled mouse brain tissue. (C) 2017 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
