Journal
OPTICS EXPRESS
Volume 25, Issue 9, Pages 10276-10286Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.25.010276
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Funding
- Natural National Science Foundation of China (NSFC) [61235012, 61620106016, 61525503, 61378091, 61405123]
- National Basic Research Program of China (Program 973) [2015CB352005]
- Guangdong Natural Science Foundation Innovation Team [2014A030312008]
- Hong Kong, Macao and Taiwan cooperation innovation platform & major projects of international cooperation in Colleges and Universities in Guangdong Province [2015KGJHZ002]
- Shenzhen Basic Research Project [JCYJ20160328144746940, JCYJ20160308093035903, JCYJ20150930104948169]
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We propose a method to improve the resolution of coherent anti-Stokes Raman scattering microscopy (CARS), and present a theoretical model. The proposed method, coherent anti-Stokes Raman scattering difference microscopy (CARS-D), is based on the intensity difference between two differently acquired images. One being the conventional CARS image, and the other obtained when the sample is illuminated by a doughnut shaped spot. The final super-resolution CARS-D image is constructed by intensity subtraction of these two images. However, there is a subtractive factor between them, and the theoretical model sets this factor to obtain the best imaging effect.
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