Journal
OPTICS EXPRESS
Volume 25, Issue 9, Pages 10368-10383Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.25.010368
Keywords
-
Categories
Funding
- W. M. Keck Foundation
- National Science Foundation [1353461, 1429810]
- Direct For Biological Sciences [1353461] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1429810] Funding Source: National Science Foundation
- Div Of Biological Infrastructure [1353461] Funding Source: National Science Foundation
Ask authors/readers for more resources
The small correction volume for conventional wavefront shaping methods limits their application in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single correction by conjugate adaptive optics and remote focusing (CAORF). The remote focusing module can maintain the conjugation between the adaptive optical (AO) element and the scattering layer during three-dimensional scanning. This new configuration provides a wider correction volume by better utilization of the memory effect in a fast three-dimensional laser scanning microscope. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We also demonstrate three-dimensional fluorescence imaging, multi-spot patterning through a scattering layer and two-photon imaging through mouse skull tissue. (C) 2017 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available