Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 29, Issue 1, Pages 1126-1136Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2018.1520203
Keywords
-blocker; immunochromatographic assay; monoclonal antibody; penbutolol; strip test
Funding
- Natural Science Foundation of Jiangsu Province
- MOF [BK20150138, 2016YFD0401101, BK20150145, CMB21S1614, CLE02N1515, 201513006]
- Taishan Industry Leading Talent Special Funds
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A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5ng/mL in 0.01 M PBS (pH 7.4) and 0.5ng/mL in pig urine. All results were obtained within 5min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB.
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