Hyperpolarized fumarate via parahydrogen

We produce hyperpolarized [1-C-13]fumarate in the proton nuclear spin singlet state by pairwise trans-addition of parahydrogen to a molecular precursor using a ruthenium-based catalyst in water. The proton singlet state is transformed into observable carbon magnetization by radiofrequency pulses to enhance the C-13 signal by a factor of 1000 using 50% para-enriched hydrogen gas.