Journal
NATURE PLANTS
Volume 4, Issue 10, Pages 792-801Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41477-018-0235-5
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Funding
- Australian Research Council Discovery Project [DP180102630]
- Australian Research Council Future Fellowships [FT160100276, FT160100218]
- Australian Research Council Discovery Early Career Researcher Award [DE170100054]
- Mizutani Foundation for Glycoscience [160151]
- DOE Joint BioEnergy Institute - U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- Australia Research Council Centre of Excellence in Plant Cell Walls [CE110001007]
- NSF-RCN [0090281]
- Australian Research Council [FT160100276, DE170100054] Funding Source: Australian Research Council
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Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational protein modifications and glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi, requiring cytosolic-derived nucleotide sugars, which need to be actively transferred into the Golgi lumen by nucleotide sugar transporters. We identified a Golgi-localized nucleotide sugar transporter from Arabidopsis thaliana with affinity for UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) and assigned it UDP-GlcNAc transporter 1 (UGNT1). Profiles of N-glycopeptides revealed that plants carrying the ugntl loss-of-function allele are virtually devoid of complex and hybrid N-glycans. Instead, the N-glycopeptide population from these alleles exhibited high-mannose structures, representing structures prior to the addition of the first GlcNAc in the Golgi. Concomitantly, sphingolipid profiling revealed that the biosynthesis of GlcNAc-containing glycosyl inositol phosphorylceramides (GIPCs) is also reliant on this transporter. By contrast, plants carrying the loss-of-function alleles affecting ROCK1, which has been reported to transport UDP-GlcNAc and UDP-N-acetylgalactosamine, exhibit no changes in N-glycan or GIPC profiles. Our findings reveal that plants contain a single UDP-GlcNAc transporter that delivers an essential substrate for the maturation of N-glycans and the GIPC class of sphingolipids.
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