Journal
ONCOLOGY REPORTS
Volume 38, Issue 2, Pages 1013-1020Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2017.5729
Keywords
microvesicles; human embryonic stem cell derived-mesenchymal stem cells; leukemia; apoptosis; autophagy
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Funding
- National Natural Science Foundation of China [81571221]
- Natural Science Foundation of Jiangsu Province [BK20151346]
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Human embryonic stem cell derived-mesenchymal stem cells (hESC-MSCs) are able to inhibit proliferation of leukemia cells. Microvesicles released from human embryonic stem cell derived-mesenchymal stem cells (hESC-MSC-MVs) might play an important part in antitumor activity. Microvesicles were isolated by ultracentrifugation and identified under a scanning electron microscopy and transmission electron microscope separately. After 48-h cocultured with hESC-MSCs and hESC-MSC-MVs, the number of K562 and HL60 was counted and tumor cell viability was measured by CCK8 assay. The expression of proteins Bcl-2 and Bax were estimated by western blotting. Transmission electron microscope and western blot analysis were adopted to evaluate the autophagy level. Results showed that both hESC-MSCs and hESC-MSC-MVs inhibited proliferation of leukemia cells in a concentration-dependent manner. hESC-MSC-MVs reduced the ratio of Bc1/Bax, enhanced the protein level of Beclin-1 and LC3-II conversion, thus upregulating autophagy and apoptosis. In conclusion, microvesicles released from human embryonic stem cell derived-mesenchymal stem cells inhibited tumor growth and stimulated autophagy and excessive autophagy might induce apoptosis.
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