Journal
ONCOLOGY REPORTS
Volume 37, Issue 6, Pages 3554-3564Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2017.5592
Keywords
miR-27a; colorectal cancer; metastasis; FAM172A; diagnosis; prognosis
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Funding
- Guangdong Natural Science Foundation [2014A030313324]
- Guangdong Natural Science Foundation
- Key Clinical Specialty Discipline Construction Program and Undergraduate Training Program for Innovation and Entrepreneurship [201512121116]
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Accumulating evidence shows that mircroRNAs (miRNAs) play a crucial role in the development of colorectal cancer. In our previous study, FAM172A was demonstrated to be a novel tumor suppressor gene in CRC. Therefore, the aim of the present study was to identify whether the miR-27a could be a diagnostic and prognostic marker and the regulatory relationships between miR-27a and FAM172A. We demonstrated high levels of miR-27a expression in tissues of patients with CRC as well as in CRC cell lines. There was a positive correlation between the levels of miR-27a and the poor overall survival of patients with CRC. Furthermore, elevated levels of miR-27a expression were associated with TNM stage and distant metastasis. Increased expression or inhibition of miR-27a promoted or inhibited the metastasis of CRC cell lines, respectively. Moreover, we showed that miR-27a directly targets the 3(1)untranslated region of FAM172A mRNA by using, a dual-luciferase assay. Increased or decreased expression of FAM172A expression was observed when miR-27a expression was inhibited or elevated in the CRC cells, respectively. In summary, our study showed that miR-27a expression is a diagnostic and prognostic marker and correlates with overall survival of patients with CRC. Therefore, it may be a therapeutic approach for preventing metastasis of CRC to inhibit expression of miR-27a or increase expression of FAM172A.
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