Journal
ONCOLOGY REPORTS
Volume 37, Issue 5, Pages 2688-2694Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2017.5527
Keywords
aptamer; activatable; probe; cancer cell; frozen cancer tissue
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Funding
- Programs for Changjiang Scholars and Innovative Research Team in University [IRT_15R13]
- National Natural Scientific Foundation of China [81430055, 81372452]
- International Cooperation Project of the Ministry of Science and Technology of China [2015DFA31320]
- Project for Innovative Research Team in Guangxi Natural Science Foundation [2015GXNSFFA139001]
- Project of Science and Technology of Guangxi [14125008-2-12, 1599005-2-10]
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It is significant to develop a probe with sensitivity and specificity for the detection of cancer cells. The present study aimed to develop an 'activatable' aptamer-based fluorescence probe (AAFP) to detect cancer cells and frozen cancer tissue. This AAFP consisted of two fragments: aptamer TLS11a that targets HepG2 cells, and two short extending complementary DNA sequences with a 5'- and 3'-terminus that make the aptamer in hairpin structure a capable quencher to fluorophore. The ability of the AAFP to bind specifically to cancer cells was assessed using flow cytometry, fluorescence spectroscopy and fluorescence microscopy. Its ability to bind to frozen cancer tissue was assessed using fluorescence microscopy. As a result, in the absence of cancer cells, AAFP showed minimal fluorescence, reflecting auto-quenching. In the presence of cancer cells, however, AAFP showed a strong fluorescent signal. Therefore, this AAFP may be a promising tool for sensitive and specific detection of cancer.
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