Chromatographic Isolation and Structural Elucidation of Secondary Metabolites from the Soil-Inhabiting Fungus Aspergillus fumigatus 3T-EGY

Eight compounds were isolated and identified from the soil-inhabiting fungus Aspergillus fumigatus 3T-EGY, namely, stearic acid (1), a-linolenic acid (2), physcion (3), di-(2-ethylhexyl) phthalate (4), 2,4,5,17-tetramethoxy pradimicin lactone (5), 3,5-dihydroxy-7-O-alpha-rhamnopyranoyl-2H-chromen-2-one (6), juglanthraquinone A-5-O-D-rhodosamine( 4 ' -> 1 '')-2-deoxy-D-glucose(4 '' -> 1 ''')-cinerulose B (7), and micropeptin (8). Their structures were determined on the basis of one-dimensional (1D-) and two-dimensional nuclear magnetic resonance (2D-NMR) [H-1-, C-13-NMR, H-1-H-1 COSY (COrrelated SpectroscopY), and H-1-C-13 HMBC (Heteronuclear Multiple Bond Correlation) spectroscopy]. Compound 7 showed moderate in vitro antimicrobial activity against three pathogenic strains with inhibition zones values were ranged from 9.0 to 10.66 mm compared to neomycin as a positive control with inhibition zones values were ranged from 14.0 to 19.0 mm.