Journal
NUCLEIC ACIDS RESEARCH
Volume 45, Issue 19, Pages 11159-11173Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx741
Keywords
-
Categories
Funding
- National Science Foundation Division of Mathematical Sciences (DMS) [1410047]
- National Institutes of Health [R37 GM32238, T32CA201159-01]
- National Science Foundation [DMS-1412844, DMS-1462992]
- Direct For Mathematical & Physical Scien
- Division Of Mathematical Sciences [1462992, 1412844] Funding Source: National Science Foundation
- Division Of Mathematical Sciences
- Direct For Mathematical & Physical Scien [1410047, 1517274] Funding Source: National Science Foundation
Ask authors/readers for more resources
Regions of highly repetitive DNA, such as those found in the nucleolus, show a self-organization that is marked by spatial segregation and frequent self-interaction. The mechanisms that underlie the sequestration of these sub-domains are largely unknown. Using a stochastic, bead-spring representation of chromatin in budding yeast, we find enrichment of protein-mediated, dynamic chromosomal cross-links recapitulates the segregation, morphology and self-interaction of the nucleolus. Rates and enrichment of dynamic crosslinking have profound consequences on domain morphology. Our model demonstrates the nucleolus is phase separated from other chromatin in the nucleus and predicts thatmultiple rDNA loci will form a single nucleolus independent of their location within the genome. Fluorescent labeling of budding yeast nucleoli with CDC14-GFP revealed that a split rDNA locus indeed forms a single nucleolus. We propose that nuclear subdomains, such as the nucleolus, result from phase separations within the nucleus, which are driven by the enrichment of protein-mediated, dynamic chromosomal crosslinks.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available