Journal
NUCLEIC ACIDS RESEARCH
Volume 45, Issue 9, Pages 5142-5152Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx103
Keywords
-
Categories
Funding
- National Science Foundation [MCB-1252370]
- European Commission (Marie Curie IOF grant) [PIOF-GA-2012-330069]
- Swedish University of Agricultural Sciences
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1252370] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1608392] Funding Source: National Science Foundation
Ask authors/readers for more resources
tRNA-derived RNA fragments (tRFs) are 18-26 nucleotide small RNAs that are not random degradation products, but are rather specifically cleaved from mature tRNA transcripts. Abundant in stressed or viral-infected cells, the function and potential targets of tRFs are not known. We identified that in the unstressed wild-type male gamete containing pollen of flowering plants, and analogous reproductive structure in non-flowering plant species, tRFs accumulate to high levels. In the reference plant Arabidopsis thaliana, tRFs are processed by Dicer-like 1 and incorporated into Argonaute1 (AGO1), akin to a microRNA. We utilized the fact that many plant small RNAs direct cleavage of their target transcripts to demonstrate that the tRF-AGO1 complex acts to specifically target and cleave endogenous transposable element (TE) mRNAs produced from transcriptionally active TEs. The data presented here demonstrate that tRFs are bona-fide regulatory microRNA-like small RNAs involved in the regulation of genome stability through the targeting of TE transcripts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available