Journal
NUCLEIC ACIDS RESEARCH
Volume 45, Issue 18, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx682
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Funding
- National Institutes of Health (NIH) [R01GM056006]
- National Science Foundation [1158254]
- Hellman Foundation
- NIH [R01GM056006]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1158254] Funding Source: National Science Foundation
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Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.
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