Journal
NUCLEAR SCIENCE AND TECHNIQUES
Volume 29, Issue 1, Pages -Publisher
SPRINGER SINGAPORE PTE LTD
DOI: 10.1007/s41365-017-0348-y
Keywords
Protmin; Nanosensor; Poly-adenine; Lead ion; Intracellular detection
Categories
Funding
- National Natural Science Foundation of China [21390414, 21605087]
- Chinese Academy of Sciences [QYZDJ-SSW-SLH031]
- China Postdoctoral Science Foundation [BX201700123]
- Scientific Research Foundation of Nanjing University of Posts and Telecommunications [NY215058]
- Natural Science Fund for Colleges and Universities in Jiangsu Province [16KJB150032]
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In this study, we designed and applied protein-mimicking nanoparticles (Protmin) as an intracellular nanosensor for in vivo detection of lead ions (Pb2+). Monodispersed gold nanoparticles (AuNPs) of 13 nm in diameter were modified using poly-adenine-tailed Pb2+ specific 8-17 DNAzyme to form a spherical and functional Protmin. Substrate strands modified with a fluorophore at the 5' end and a quencher at the 3' end were bound to DNAzyme. Pb2+ facilitated cleavage of DNAzyme to release the fluorophore-modified short strands to generate fluorescence. We observed rapid kinetics of the Protmin nanosensor, for which the typical assay time was 10 min. Further, we demonstrated the Protmin nanosensor could readily enter living cells and respond to Pb2+ in the intracellular environment. The broad of range of Protmin designs will be useful for advancing biological and medical applications.
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