Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 25, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12929-018-0494-5
Keywords
Extracellular vesicles exosomes; Microvesicles; Imaging; Biodistribution; Fluorescence; Bioluminescence; MRI; SPECT; Dyes
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Funding
- Ministry of Science and Technology (MOST) [104-2320-B-007-005-MY2, 106-2320-B-007-004-MY3]
- Academia Sinica Innovative Materials and Analysis Technology Exploration (i-MATE) Program [AS-iMATE-107-33]
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Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by cells. They range from 30nm to several micrometers in diameter, and ferry biological cargos such as proteins, lipids, RNAs and DNAs for local and distant intercellular communications. EVs have since been found to play a role in development, as well as in diseases including cancers. To elucidate the roles of EVs, researchers have established different methods to visualize and study their spatiotemporal properties. However, since EV are nanometer-sized, imaging them demands a full understanding of each labeling strategy to ensure accurate monitoring. This review covers current and emerging strategies for EV imaging for prospective studies.
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