Journal
ENDOCRINE-RELATED CANCER
Volume 22, Issue 4, Pages 505-517Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/ERC-15-0105
Keywords
ER alpha; H19; luminal progenitors; ER vertical bar breast cancer cells
Categories
Funding
- CancerCare Manitoba Foundation [761099745]
- University of Manitoba [125972]
- Manitoba Health Research Council [125348]
- Canadian Breast Cancer Foundation (CBCF-Prairies/NWT) [76-109-9750, 1213, 1301]
- Canadian Cancer Society Research Institute
- Manitoba Health Research Institute
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Although the role of estrogen signaling in breast cancer development has been extensively studied, the mechanisms that regulate the indispensable role of estrogen in normal mammary gland development have not been well studied. Because of the unavailability of culture system to maintain estrogen-receptor-positive (ER alpha(+)) cells in vitro, the molecular mechanisms that regulate estrogen/ER alpha signaling in the normal human breast are unknown. In the present study, we examined the effects of estrogen signaling on ER alpha(+) human luminal progenitors using a modified matrigel assay and found that estrogen signaling increased the expansion potential of these progenitors. Furthermore, we found that blocking ER alpha attenuated luminal progenitor expansion and decreased the luminal colony-forming potential of these progenitors. Additionally, blocking ER alpha decreased H19 expression in the luminal progenitors and led to the development of smaller luminal colonies. We further showed that knocking down the H19 gene in the luminal progenitors significantly decreased the colony-forming potential of the luminal progenitors, and this phenotype could not be rescued by the addition of estrogen. Lastly, we explored the clinical relevance of the estrogen-H19 signaling axis in breast tumors and found that ER alpha(+) tumors exhibited a higher expression of H19 as compared with ER alpha-tumors and that H19 expression showed a positive correlation with ER alpha expression in those tumors. Taken together, the present results indicate that the estrogen-ER alpha-H19 signaling axis plays a role in regulating the proliferation and differentiation potentials of the normal luminal progenitors and that this signaling network may also be important in the development of ER+ breast cancer tumors.
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