Journal
NEW JOURNAL OF PHYSICS
Volume 19, Issue -, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1367-2630/aa7b0f
Keywords
dynamic light scattering; multicellular structures; coherent optics
Categories
Funding
- LABEX WIFI (Laboratory of Excellence) [ANR-10-LABX-24, ANR-10-IDEX-0001-02 PSL*]
- Agence Nationale pour la Recherche [ANR-13-BSV5-0008-447 01]
- Institut National de la Sante et de la Recherche Medicale [2011-1-PL BIO-448 11-IC-1]
- Centre National de la Recherche Scientifique ('Programme Emergence')
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Determining the structure and the internal dynamics of tissues is essential to understand their functional organization. Microscopy allows for monitoring positions and trajectories of every single cell. Those data are useful to extract statistical observables, such as intercellular distance, tissue symmetry and anisotropy, and cell motility. However, this procedure requires a large and supervised computational effort. In addition, due to the large cross-section of cells, the light scattering limits the use of microscopy to relatively thin samples. As an alternative approach, we propose to take advantage of light scattering and to analyze the dynamical diffraction pattern produced by a living tissue illuminated with coherent light. In this article, we illustrate with a few examples that supra-cellular structures produce an exploitable diffraction signal. From the diffraction signal, we deduce the mean distance between cells, the anisotropy of the supra-cellular organization and, from its fluctuations, the mean speed of moving cells. This easy to implement technique considerably reduces analysis time, allowing real time monitoring.
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