Journal
NEW BIOTECHNOLOGY
Volume 34, Issue -, Pages 54-58Publisher
ELSEVIER
DOI: 10.1016/j.nbt.2016.05.002
Keywords
Pachysolen tannophilus; Poly(vinyl alcohol) cryogel; Crude glycerol; Ethanol; Immobilised cells
Funding
- Presidium of RAS (Program of basic scientific research) [25]
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Screening among naturally occurring yeast strains of Pachysolen spp. that are capable of producing ethanol from glycerol under aerobic conditions identified the most active culture, P. tannophilus Y-475. Conversion of glycerol by this producer immobilised in poly(vinyl alcohol) cryogel resulted in a 90% yield of ethanol relative to the theoretical limit. The maximum rate of alcohol accumulation was 0.64 +/- 0.01 g L(-1)h(-1) at a 25 g L-1 concentration of glycerol in the culture medium. We demonstrated the efficacy of reusing immobilised cells (for a minimum of 16 working cycles for batch mode of crude glycerol conversion to ethanol) and the possibility of long-term (for a minimum of 140 h) use of the cells in continuous mode with a maximum process productivity of 0.63 +/- 0.02 g L-1 h(-1), while the medium dilution rate in the reactor was 0.062 +/- 0.001 h(-1). Reduction of metabolic activity did not exceed 5-7% relative to baseline. Immobilised cells were demonstrated to withstand long-term storage in frozen form for at least 2 years while retaining high metabolic activity. (C) 2016 Elsevier B.V. All rights reserved.
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