Journal
BIOMOLECULES
Volume 8, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/biom8040130
Keywords
Candida glabrata; MNN2 gene; antifungal; resistance; biofilm matrix
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2013, POCI-01-0145-FEDER-006684]
- European Regional Development Fund under Norte2020-Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004]
- European Regional Development Fund [SFRH/BD/93078/2013]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/93078/2013] Funding Source: FCT
Ask authors/readers for more resources
Candida glabrata biofilms are recognized to have high resistance to antifungals. In order to understand the effect of mannans in the resistance profile of C. glabrata mature biofilms, C. glabrata Delta mnn2 was evaluated. Biofilm cell walls were analysed by confocal laser scanning microscopy (CLSM) and their susceptibility was assessed for fluconazole, amphotericin B, caspofungin, and micafungin. Crystal violet and Alcian Blue methods were performed to quantify the biomass and the mannans concentration in the biofilm cells and matrices, respectively. The concentration of beta-1,3 glucans was also measured. No visible differences were detected among cell walls of the strains, but the mutant had a high biomass reduction, after a drug stress. When compared with the reference strain, it was detected a decrease in the susceptibility of the biofilm cells and an increase of beta-1,3 glucans in the C. glabrata Delta mnn2. The deletion of the MNN2 gene in C. glabrata induces biofilm matrix and cell wall variabilities that increase the resistance to the antifungal drug treatments. The rise of beta-1,3 glucans appears to have a role in this effect.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available