4.4 Article

Electroacupuncture preconditioning and postconditioning inhibit apoptosis and neuroinflammation induced by spinal cord ischemia reperfusion injury through enhancing autophagy in rats

Journal

NEUROSCIENCE LETTERS
Volume 642, Issue -, Pages 136-141

Publisher

ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2017.02.010

Keywords

Electroacupuncture; Autophagy; Spinal cord ischemia reperfusion injury; Apoptosis; Neuroinflammation

Categories

Funding

  1. Natural Science Foundation of China [81401000, 81601053]
  2. Doctoral Research Fund of Liaoning Province [20141035]

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Electroacupuncture (EA) has beneficial effects on spinal cord ischemia reperfusion (I/R) injury, but the underlying mechanisms are not fully understood. This study aimed to investigate the role of autophagy in the protection of EA preconditioning and postconditioning against spinal cord I/R injury. For this, spinal cord I/R injury was induced by 14 min occlusion of the aortic arch, and rats were treated with EA for 20 min before or after the surgery. The expression of autophagy components, light chain 3 and Beclin 1, was assessed by Western blot. The hind-limb motor function was assessed using the Basso-Beattie-Bresnahan (BBB) criteria, and motor neurons in the ventral gray matter were counted by histological examination. The apoptosis of neurocyte was assessed by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. The expression of tumor necrosis factor-alpha (TNT-alpha), interleukin-1 beta (IL-1 beta), and matrix metalloproteinase-9 (MMP-9) was also measured using Western blot or enzyme-linked immunosorbent assay (ELISA). Either EA preconditioning or post conditioning enhanced autophagy, and minimized the neuromotor dysfunction and histopathological deficits after spinal cord I/R injury. In addition, EA suppressed I/R-induced apoptosis and increased in the expression of TNF-alpha, IL-1 beta, and MMP-9. In contrast, the autophagic inhibitor (3-methyladenine, 3-MA) inhibited the neuroprotective effects of EA. Moreover, 3-MA increased the apoptosis and the expression of TNF-alpha, IL-1 beta, and MMP-9. In summary, these findings suggested that EA preconditioning and postconditioning could alleviate spinal cord I/R injury, which was partly mediated by autophagy upregulation-induced inhibition of apoptosis and neuroinflammation. (C) 2017 Elsevier B.V. All rights reserved.

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