OLEOCANTHAL AMELIORATES AMYLOID-β OLIGOMERS' TOXICITY ON ASTROCYTES AND NEURONAL CELLS: IN VITRO STUDIES

Extra-virgin olive oil (EVOO) has several health promoting effects. Evidence have shown that EVOO attenuates the pathology of amyloid-beta (A beta) and improves cognitive function in experimental animal models, suggesting it's potential to protect and reduce the risk of developing Alzheimer's disease (AD). Available studies have linked this beneficial effect to oleocanthal, one of the active components in EVOO. The effect of oleocanthal against AD pathology has been linked to its ability to attenuate A beta and tau aggregation in vitro, and enhance A beta clearance from the brains of wild-type and AD transgenic mice in vivo. However, the ability of oleocanthal to alter the toxic effect of A beta on brain parenchymal cells is unknown. In the current study, we investigated oleocanthal effect on modulating A beta oligomers (A beta o) pathological events in neurons and astrocytes. Our findings demonstrated oleocanthal prevented A beta o-induced synaptic proteins, SNAP-25 and PSD-95, down-regulation in neurons, and attenuated A beta o-induced inflammation, glutamine transporter (GLT1) and glucose transporter (GLUT1) down-regulation in astrocytes. A beta o-induced inflammation was characterized by interleukin-6 (IL-6) increase and glial fibrillary acidic protein (GFAP) upregulation that were reduced by oleocanthal. In conclusion, this study provides further evidence to support the protective effect of EVOO-derived phenolic secoiridoid oleocanthal against AD pathology. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.