Journal
NEUROREPORT
Volume 28, Issue 17, Pages 1127-1133Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0000000000000877
Keywords
acetone-ether-water model; chloroquine; itch; Mas-related G protein-coupled receptor A3
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Funding
- National Natural Science Foundation of China [31271181, 31328012, 81403407]
- Natural Science Foundation of Jiangsu Province [BK20151571]
- Hunan Cooperative Innovation Center for Molecular Target New Drug Study
- Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor - 'Qing Lan Project' in Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions (Integration of Traditional Chinese and Western Medicine)
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Chronic itch, a distressing symptom of many cutaneous and systemic diseases, significantly impairs quality of life. However, its underlying molecular mechanism is still unclear. Mas-related G protein-coupled receptor A3 (MrgprA3) is considered an itch-specific receptor. MrgprA3(+) neurons are identified as a class of itch-specific neurons, but the role of MrgprA3 in chronic itch remains elusive. An acetone-ether-water (AEW) model as a histamine-independent itch model is often used in the study of chronic pruritus. In this study, behavioral tests, immunostaining, cell culture, calcium imaging, and other experiments were carried out to examine the expression of MrgprA3. The results showed that the scratching bouts induced by chloroquine increased significantly under the AEW condition; the density of MrgprA3(+) sensory fibers in the AEW-treated skin area and the number of MrgprA3(+) neurons in dorsal root ganglia from the AEW model mice also increased significantly. Further analysis showed that the MrgprA3 in mRNA level was also increased after AEW treatment. These results indicated that MrgprA3 played a crucial role in chronic pruritus in the AEW model. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.
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