Living cell imaging and sensing of hydrogen sulfide using high-efficiency fluorescent Cu-doped carbon quantum dots

In this paper, we have presented a convenient strategy to fabricate Cu-doped carbon quantum dots (Cu-CQDs) for H2S sensing and imaging applications. Cu-CQDs are obtained by one step hydrothermal carbonization, using ethylenediaminetetraacetic acid salts and cupric chloride as sources, which simultaneously incorporates Cu(H2S-responding site) and a luminescent carbon quantum dot (fluorophore). Thus, the added NaHS (H2S donor) can lead to the fluorescence quenching of Cu-CQDs, and such a fluorescence response allows the detection of H2S in the range of 2-500 mu M with a detection limit of 0.5 mu M (S/N = 3). In addition, confocal imaging was achieved successfully in living cells.