4.4 Article

Inflammatory cell distribution in colon mucosa as a new tool for diagnosis of irritable bowel syndrome: A promising pilot study

Journal

NEUROGASTROENTEROLOGY AND MOTILITY
Volume 30, Issue 1, Pages -

Publisher

WILEY
DOI: 10.1111/nmo.13223

Keywords

inflammatory cells; irritable bowel syndrome; low-grade inflammation; macrophages; mast cells

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BackgroundCurrently, there are no histological criteria to diagnose irritable bowel syndrome (IBS). Our aims were (i) to examine the distribution of inflammatory cells in the colon of healthy and IBS subjects and (ii) to find histological diagnosis criteria for IBS. MethodsColonic biopsies were taken from four distinct regions of the colon from 20 controls (HC) and 11 patients with IBS (4 with constipation (IBS-C) and 7 with diarrhea (IBS-D) and embedded in paraffin. Macrophages, mast cells, eosinophils, and T lymphocytes were immunostained and positive cells counted. Key ResultsIn both HC and IBS patients, global cellularity decreased from the cecum to the rectum (P<.01) which is attributed to reduced number of macrophages (P<.05) and eosinophils (P<.001) but not T cells. Mast cells were reduced in IBS (P<.05) but not in HC, particularly in IBS-D (P<.05). Results showed higher number of macrophages in the left colon of IBS subjects than HC (P<.05). Conclusion & InferencesHere we report a decreasing gradient of immune cells from the cecum to the rectum of the human colon. Although global cellularity cannot be used to distinguish between IBS and HC, closer analysis of macrophages and mast cells may be useful markers to confirm IBS histologically and to differentiate between IBS-C and IBS-D when clinical presentation alternates between constipation and diarrhoea. This pilot study remains to be confirmed with greater number of patients.

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