Journal
NEUROCHEMISTRY INTERNATIONAL
Volume 103, Issue -, Pages 24-35Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2016.12.006
Keywords
P2X(7) receptor; Dorsal root ganglia; Satellite glial cell; Resveratrol; Neuropathic pain
Categories
Funding
- National Natural Science Foundation of China [31560276, 81570735, 81460200, 81171184, 31060139, 30860086, 81200853]
- Jiangxi Province Gan Po Excellence Talent 555 project leading talent project from Technology Pedestal and Society Development Project of Jiangxi Province [20151122040105]
- Natural Science Foundation of Jiangxi Province [20142BAB205028, 20142BAB215027]
- Educational Department of Jiangxi Province [GJJ13155, GJJ14319]
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The P2X(7) receptor mediates afferent nerve activation and is related to chronic neuropathic pain. Resveratrol (RES) has also been reported to exhibit anti-inflammatory effects. In this study, we investigated the neuroprotective effect of RES on the transmission of neuropathic pain mediated by the P2X(7) receptor. The P2X(7) mRNA and protein expression levels in L4-L5 dorsal root ganglia (DRG)s of the chronic constriction injury (CCI) group were significantly higher than those observed in the Ctrl + NS, Sham + RES and Sham groups. RES increased the threshold of thermal and mechanical hypersensitivity in rats with chronic neuropathic pain. The P2X(7) mRNA and protein expression levels in the CCI + RES group were decreased compared with those in the CCI group. Our results showed that RES inhibited the upregulated co-expression of P2X(7) and glial fibrillary acidic protein (GFAP, a marker of satellite glial cells) in satellite glial cells of DRG in the CCI group. The results demonstrated that the expression of GFAP was increased in the CCI group and that RES inhibited the upregulated expression of GFAP in the rats in the CCI group. In addition, the phosphorylation levels of p38 and extracellular regulated protein kinases (ERK)1/2 in the CCI group were markedly higher than those observed in the Ctrl + NS, Sham + RES and Sham groups, whereas the phosphorylation levels of p38 and ERK1/2 in CCI + RES group were markedly lower than those observed in the CCI group. RES inhibited BzATP-activated currents in DRG non-neurons in the CCI rats. Our data provide evidence that RES may suppress the transmission of neuropathic pain mediated by the P2X(7) receptor in the satellite glial cells of dorsal root ganglia. (C) 2016 Elsevier Ltd. All rights reserved.
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