Journal
NEUROCHEMISTRY INTERNATIONAL
Volume 108, Issue -, Pages 282-286Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2017.04.017
Keywords
IRAS/nischarin; mu-Opioid receptor; Opiate dependence; Imidazoline receptors
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Funding
- Ministerio de Economia y Competitividad [SAF2011-29918]
- Fondo Europeo de Desarrollo Regional (FEDER), Spain
- Redes Tematicas de Investigacion Cooperativa en Salud-Red de Trastornos Adictivos (RETICS-RTA), Institute de Salud Carlos III (ISCIII, MINECO/FEDER), Spain [RD12/0028/0011]
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Imidazoline receptor antisera-selected (IRAS)/nischarin, a putative I-1-imidazoline receptor, has recently been shown to regulate mu-opioid receptor (OR) trafficking and resensitisation. To study a possible involvement of this mu-OR regulator in opiate dependence, the present study assessed by Western blot analysis the contents of IRAS/nischarin and mu-OR in total homogenates and subcellular preparations of postmortem human prefrontal cortex (PFC/BA9) of long-term opiate and mixed opiate/cocaine abusers as well as of matched healthy control subjects. In the PFC/BA9 of long-term opiate/cocaine abusers (all subjects together) IRAS/nischarin content was increased (+67%, p < 0.01, n = 11) when compared with matched controls (n = 10). Similar increases were found for the subgroups of opiate (+72%, n = 6) and mixed opiate/cocaine (+61%, n = 5) abusers. IRAS/nischarin immunocontents were also found increased in subcellular membrane preparations (+61%, p < 0.05, n = 10) of PFC/BA9 from opiate addicts. In the same brain samples, the levels of mu-OR were not different to those in control subjects. Based on the increased contents in brains of opiate abusers and the reported function as mu-OR regulator, IRAS/nischarin could represent a new promising target for treatment of opiate use disorder. (C) 2017 Elsevier Ltd. All rights reserved.
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