Journal
BIO-PROTOCOL
Volume 8, Issue 4, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2728
Keywords
Auxin; Degron; AID; Conditional knockdown; Protein regulation; Parasite; Toxoplasma gondii; CRISPR
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Funding
- NIAID NIH HHS [R01 AI034036, R01 AI118426] Funding Source: Medline
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Toxoplasma gondii is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding T. gondii's 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are needed to functionally investigate hundreds of putative essential protein coding genes. Accordingly, we recently implemented the auxin-inducible degron (AID) system for studying essential proteins in T. gondii. Here we provide a step-by-step protocol for examining protein function in T. gondii using the AID system in a tissue culture setting.
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