Journal
BIO-PROTOCOL
Volume 8, Issue 17, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2989
Keywords
PTZ; C. elegans; NMJ; GABA; Convulsions; Synaptic transmission
Categories
Funding
- Council of Scientific and Industrial Research (CSIR)
- DBT-Wellcome Trust India Alliance [IA/12/500516]
Ask authors/readers for more resources
Pentylenetetrazole (PTZ) is a GABAA receptor antagonist and is used to monitor presynaptic defects in the release of the inhibitory neurotransmitter GABA. PTZ is a competitive inhibitor of GABA, and prevents binding of GABA on the GABAA receptors present on the surface of muscle. In the absence of GABA binding, the excitatory to inhibitory signal ratio increases resulting in a convulsive phenotype. This assay provides a fast and reliable method to detect presynaptic defects in GABAergic synaptic transmission. The assay is based on correlating the extent of convulsions with the degree of presynaptic GABA release defects.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available