Journal
BIO-PROTOCOL
Volume 8, Issue 17, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2994
Keywords
Cell synchronization; Cell cycle; Thymidine; DNA synthesis; DNA replication
Categories
Funding
- NIH/NCI [R01CA193828, R01CA136534, R01CA200905]
- Winship Research Pathology and Integrated Cellular Imaging shared resource
- Emory Comprehensive Glycomics Core (ECGC) - Winship Cancer Institute of Emory University [P30CAJ 38292]
- Winship Fashion a Cure Research Scholar Award
- Winship Endowment Fund
- Winship
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Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.
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