Journal
BIO-PROTOCOL
Volume 8, Issue 7, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2800
Keywords
C. elegans; ROS; Mitochondria; DCF; Oxidative stress; CM-H(2)DCFDA
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Funding
- National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 OD010440]
- Alzheimer's Association [NIRG-09-132122]
- NIH [GM088213]
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Oxidative stress is implicated in the pathogenesis of various neurodegenerative diseases, including Alzheimer's disease. Oxidative stress is a result of a disruption of the equilibrium between antioxidants and oxidants, in favor of oxidants. Since mitochondria are major sites of production and reduction of reactive oxygen species (ROS), measurement of ROS levels can help us determine if mitochondrial functional integrity has been compromised. In this protocol, we describe a method to measure the level of ROS in the nematode Caenorhabditis elegans, using chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H(2)DCFDA).
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