Journal
BIO-PROTOCOL
Volume 8, Issue 11, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2863
Keywords
Rice; RYMV; Inoculation; Resistance; ELISA; Symptoms
Categories
Funding
- Global Rice Science Partnership (GRiSP) as a New Frontiers Research program (project MENERGEP)
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Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and characterization of resistance sources. This paper describes a protocol for mechanical inoculation of rice seedlings with RYMV and two methods of resistance evaluation - one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA).
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