Journal
CRISPR JOURNAL
Volume 1, Issue 1, Pages 65-74Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/crispr.2017.0010
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Funding
- Agriculture and Food Research Initiative Competitive Grants from the USDA National Institute of Food and Agriculture [2017-67007-25932]
- Bill and Melinda Gates Foundation [BMGF: 01511000146]
- NIFA [2017-67007-25932, 914499] Funding Source: Federal RePORTER
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The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to modify multiple genomic regions simultaneously controlling different agronomic traits in crops. We applied the MGE construct built by combining the tandemly arrayed tRNA-gRNA units to generate heritable mutations in the TaGW2, TaLpx-1, and TaMLO genes of hexaploid wheat. The knockout mutations generated by this construct in all three homoeologous copies of one of the target genes, TaGW2, resulted in a substantial increase in seed size and thousand grain weight. We showed that the non-modified gRNA targets in the early generation plants can be edited by CRISPR-Cas9 in the following generations. Our results demonstrate that transgenerational gene editing activity can serve as the source of novel variation in the progeny of CRISPR-Cas9-expressing plants and suggest that the Cas9-inducible trait transfer for crop improvement can be achieved by crossing the plants expressing the gene editing constructs with the lines of interest.
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