4.1 Article Data Paper

Data on preparation and characterization of an insect odorant receptor based biosensor

Journal

DATA IN BRIEF
Volume 21, Issue -, Pages 2142-2148

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.dib.2018.11.018

Keywords

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Funding

  1. Kiwi Innovation Network (KiwiNET) inaugural Emerging Innovator Award
  2. Better Border Biosecurity (B3) grant [P/321022/06]
  3. New Zealand Institute for Plant & Food Research Limited (PFR) SSIF grant [P/321061/02]
  4. Royal Society Rutherford Post-Doctoral Scholarship [RFT-PAF1501-PD]
  5. Marsden Fund grant [PAF1503]
  6. PFR SSIF grant
  7. B3 grant

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Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants [1]. (C) 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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