Journal
ACS APPLIED NANO MATERIALS
Volume 1, Issue 7, Pages 3091-3097Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.8b00933
Keywords
enzymes; DNA; phosphotriesterase; nanoparticle; quantum dot; cell free; synthetic biology; organophosphate hydrolase; bioremediation
Funding
- Office of Naval Research (ONR)
- NRL-Nanosciences Institute
- United States Department of Agruculture [2016-67021-25038]
- Naval Research Laboratory (NRL)
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Cell-free synthetic biology relies on optimally exploiting enzymatic activity, and recent demonstrations that nanoparticle (NP) and DNA scaffolding can enhance enzyme activity suggest new avenues toward this. A modular architecture consisting of a DNA cage displaying semiconductor quantum dots (QDs) that, in turn, ratiometrically display the organophosphate hydrolase phosphotriesterase (PTE) was utilized as a model system. Increasing DNA cage concentration relative to QD-PTE and creating a dense composite enhanced PTE rates up to 12.5-fold, suggesting strong synergy between the NP and DNA components; this putatively arises from increased enzymatic stability and alleviation of its rate-limiting step. Such bioinorganic composites may offer new scaffolding approaches for synthetic biology.
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