Journal
NATURE NANOTECHNOLOGY
Volume 12, Issue 4, Pages 368-377Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2016.284
Keywords
-
Funding
- Burroughs Wellcome Fund at the Scientific Interface (CASI)
- Simons Foundation
- BBRF
- Beckman Foundation
- National Science Foundation (NSF)
- Japan Society for the Promotion of Science
- Naito Foundation
- NSF GRFP fellowship
- LAM research fellowship
- Hertz Foundation
- Department of Defense
- NIH Medical Scientist Training Program [T32GM007753]
- National Institutes of Health [DP2 OD008435, P50 GM098792]
- Office of Naval Research [N00014-13-1-0424]
- NSF [MCB-1350625]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1350625] Funding Source: National Science Foundation
- Grants-in-Aid for Scientific Research [15K21770] Funding Source: KAKEN
Ask authors/readers for more resources
A distinct advantage of nanosensor arrays is their ability to achieve ultralow detection limits in solution by proximity placement to an analyte. Here, we demonstrate label-free detection of individual proteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber, measuring protein efflux from single organisms in real time. The array is fabricated using non-covalent conjugation of an aptamer-anchor polynucleotide sequence to near-infrared emissive single-walled carbon nanotubes, using a variable chemical spacer shown to optimize sensor response. Unlabelled RAP1 GTPase and HIV integrase proteins were selectively detected from various cell lines, via large near-infrared fluorescent turn-on responses. We show that the process of E. coli induction, protein synthesis and protein export is highly stochastic, yielding variability in protein secretion, with E. coli cells undergoing division under starved conditions producing 66% fewer secreted protein products than their non-dividing counterparts. We further demonstrate the detection of a unique protein product resulting from T7 bacteriophage infection of E. coli, illustrating that nanosensor arrays can enable real-time, single-cell analysis of a broad range of protein products from various cell types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available