Journal
NATURE METHODS
Volume 14, Issue 9, Pages 869-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4337
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Funding
- National Institute of Biomedical Imaging and Bioengineering
- National Institute of Heart, Lung, and Blood
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- National Key Basic Research (973) Program of China [2015CB755502]
- National Natural Science Foundation of China [81471702]
- Shenzhen Science and Technology Innovation Committee [KQCX20120816155352228]
- NIH [R01MH107238]
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We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 +/- 10 nm laterally, 729 +/- 39 nm axially) at depths similar to 250 mu m from the coverslip surface.
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