Journal
NATURE METHODS
Volume 14, Issue 10, Pages 983-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.4405
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Funding
- NIH [R01GM080139, P01NS092525, P41GM103832]
- Ovarian Cancer Research Fund
- Singapore Ministry of Education
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Cellular electron cryotomography offers researchers the ability to observe macromolecules frozen in action in situ, but a primary challenge with this technique is identifying molecular components within the crowded cellular environment. We introduce a method that uses neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction. Subsequent subtomogram classification and averaging yield in situ structures of molecular components of interest. The method is available in the EMAN 2.2 software package.
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